Antibacterial Activity of Southern African Members of the Combretaceae
Based on the results obtained with Combretum erythrophyllum, other members of
the Combretaceae were examined to find the best source for isolating antibacterial
compounds. Leaves of 27 species of Combretum, Terminalia, Pteleopsis, and Quisqualis
were collected, dried, milled, and extracted with acetone. The MIC of extracts
was determined by our microplate serial dilution technique using Staphylococcus
5.12 Results on Antibacterial Activity Obtained with Members of the Combretaceae 109
aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli as test or-
ganisms. All extracts inhibited the growth of the four test isolates with MIC values
generally between 0.1 and 6 mg mL–1 and an average of 2.01 mg mL–1. After stor-
ing extracts for six weeks at 7 °C there was a slight loss of activity with MIC values
increasing from 1.75 mg mL–1 to 2.24 mg mL–1. The Gram-positive strains were
slightly more sensitive with an average MIC of 1.8 mg mL–1 than the Gram-nega-
tive strains with an MIC of 2.22 mg mL–1. Based on the MIC values and the total
content in each plant, the seven plants with the highest antibacterial activity were
C. molle, C. petrophilum, C. moggii, C. erythrophyllum, C. padoides, C. paniculatum,
and C. mossambicense [21].
Based on the results obtained with Combretum erythrophyllum, other members of
the Combretaceae were examined to find the best source for isolating antibacterial
compounds. Leaves of 27 species of Combretum, Terminalia, Pteleopsis, and Quisqualis
were collected, dried, milled, and extracted with acetone. The MIC of extracts
was determined by our microplate serial dilution technique using Staphylococcus
5.12 Results on Antibacterial Activity Obtained with Members of the Combretaceae 109
aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli as test or-
ganisms. All extracts inhibited the growth of the four test isolates with MIC values
generally between 0.1 and 6 mg mL–1 and an average of 2.01 mg mL–1. After stor-
ing extracts for six weeks at 7 °C there was a slight loss of activity with MIC values
increasing from 1.75 mg mL–1 to 2.24 mg mL–1. The Gram-positive strains were
slightly more sensitive with an average MIC of 1.8 mg mL–1 than the Gram-nega-
tive strains with an MIC of 2.22 mg mL–1. Based on the MIC values and the total
content in each plant, the seven plants with the highest antibacterial activity were
C. molle, C. petrophilum, C. moggii, C. erythrophyllum, C. padoides, C. paniculatum,
and C. mossambicense [21].
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